Scientific lab report of enzyme catalysis
Effect of enzyme concentration on enzyme activity lab report
Remember to gently swirl the solution after adding each drop. Inhibitors work by unfolding or destabilizing bonds, thus denaturing the enzyme. Mix this solution well. In addition to observing enzyme catalysis, this lab examines how different factors affect enzyme activity. A magnetic stirring was placed into the beaker, which was in turn placed on a hot plate. Because of enzyme activity, cells can carry out complex chemical activities at relatively low temperatures. Enzymes have active sites, in which only a particular substrate can bind onto. Using a clean and unused pipet, we then added 1mL of the boiled catalase after 5 minutes to the unlabeled cup and observed. Results: 1. If the potato had been boiled, the reaction would not have occurred as quickly as it had without any disruptions in environment. Add 10 mL of H2SO4 1. Exercise 2C The materials needed for exercise 2C of the lab are: 20 mL of 1. To test the effect of varying pH, I would create a controlled experiment where the only thing that is different is the pH level, which would be the independent value.
While waiting, we rinsed our unlabeled cup and used the H2O2 to transfer 10mL of H2O2 to the rinsed unlabeled cup. If it becomes too cold, the enzyme will denature and it will no longer function properly.
The catalase might have mixed with ice water, it might have come into contact with another substance, been contaminated, etc. This makes up for the exceptionally high rate of reaction. Another example is lots of product with a little enzyme forms more substrate. After left overnight, only 0.
The boiling catalase slowed down the reaction between H2O2 and the catalase because of the raise in temperature. The following calculations were made: 0.
based on 101 review